Urine Protein Electrophoresis without Sample Concentration: An Evaluation of Two Methods
نویسندگان
چکیده
منابع مشابه
Proteins of human urine. I. Concentration and analysis by two-dimensional electrophoresis.
Human urine contains many different proteins, all in trace amounts, and evaluation of some of them is diagnostically useful. Two-dimensional electrophoretic methods have been developed for resolving complex mixtures (1-3); thus it has become of interest to examine in detail the urinary proteins that normally are present, and those that vary, appear, or disappear in disease. The development of m...
متن کاملFalse-negative urine protein electrophoresis by semiautomated gel electrophoresis.
Urine is evaluated by electrophoretic methods both to gather information about the location and degree of damage within the nephron and to detect and quantify monoclonal free light chains (Bence Jones proteins). For many years, urine protein electrophoresis consisted of concentrating the urine (usually 50-fold) followed by manual application of the concentrated sample to a gel. Recently, semiau...
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There is a great diversity of protein samples types and origins, therefore the optimal procedure for each sample type must be determined empirically. In order to obtain a reproducible and complete sample presentation which view as many proteins as possible on the desired 2DE gel, it is critical to perform additional sample preparation steps to improve the quality of the final results, y...
متن کاملAlternative methods for newborn urine sample collection.
PURPOSE To investigate the accuracy of laboratory results of urine samples collected from cotton balls as compared with paired samples collected from catheterization, Preemie Pampers, and Newborn Pampers. METHOD Urine samples were collected from 30 infants by aspirating urine from a cotton ball placed over the meatus. An additional urine sample was obtained from the same infants by aspiration...
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To the Editor: In a recent report, Keren et al. (1 ) described a case in which the Sebia semiautomated electrophoresis method failed to demonstrate proteinuria in a 50-fold concentrated urine sample despite the presence of 2 g/24 h total protein. The reason for this failure was attributed to the presence of debris associated with cellular elements that interfered with the wicking of the urine i...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1993
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456329303000316